This chapter of the book Introduction in Enzyme Technology, K.-E. Jäger, A. Liese, C. Syldaktk (eds.) describes the use of enzymes in biosensors. Enzyme-based biosensors have enjoyed a prosperous growth market for more than five decades and are increasingly being used in biotechnological processes. Typically, they comprise a biological recognition element together with a physicochemical transducer. The main advantages of biosensors are their easy-to-use operation, cost-effective manufacturing, and their ability for sensitive and highly accurate detection of specific analytes. A brief overview on “biosensor technology” is given, including relevant sensor parameters, followed by major developments in electrochemical enzyme biosensors with a more detailed focus on possible applications in the field of biotechnology. Looking beyond the “end of one’s nose” will offer insight into alternative transducer principles and biomolecules.
Schöning, M.J., Poghossian, A., Enzymes and Biosensor Technology, in K.E. Jäger, A. Liese, C. Syldatk (eds.) Introduction to Enzyme Technology, Springer Spektrum, Berlin (Germany), 2024, ISBN 978-3-031-42998-9, pp 385-416.